Phosphate solubilizing microorganisms principles and. Mechanisms of inorganic phosphate solubilization by psm. Role of phosphate solubilizing fungi and microbes for. Effectiveness of a rock phosphate solubilizing fungus to. Effect of phosphate solubilizing fungi on growth and. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan b present address. Pvk medium to represent soil phosphate pool and a rock. Phosphatesolubilizing microorganisms are ubiquitous, whose numbers vary from soil to soil.
After screening, 14 fungal isolates were obtained, which were further screened based. Isolation and characterization of phosphatesolubilizing. Lastly, the role of phosphate solubilizing microorganisms in aerobic rice cultivation is highlighted. Phosphatesolubilizing microorganisms associated with the. Because of the negative charge of phosphate ions, they are quickly absorbed after weathering of clays or detritus particles, forming insoluble forms of aluminum. Sep 12, 2007 a total of 32 bacterial isolates including mesorhizobium n10, azotobacter n12 and phosphate solubilizing bacteria n10 were isolated and tested for siderophore, hcn, ammonia, indole acetic acid production and phosphate solubilization in vitro. Phosphatesolubilizing bacteria employ different strategies to convert unavailable forms of phosphate into available forms. The focus is on understanding both the basic and applied aspects of phosphate solubilizing microorganisms and how phosphorusdeficient soils can be transformed into phosphorusrich ones by applying phosphate solubilizing microorganisms. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Microbial solubilization of fixed mineral phosphates in the soil is an important process in natural ecosystems and in agricultural soils. Evaluation of phosphate solubilizing fungi from mangrove soil. After 5 days, serial dilution of the enriched medium was carried out and.
Xiaoa cq, chib ra, huangb xh, zhangc wx, qiua gz, wanga dz 2008 optimization for rock phosphate solubilization by phosphatesolubilizing fungi isolated from phosphate mines. Pdf lignolytic and phosphate solubilizing efficiency of. Phosphorus is abundant in soils in both organic and inorganic forms. Inoculation of phosphate solubilizing fungi and mycorrhizal fungi improves the physiochemical, biochemical and biological properties of rockp amended soil. Phosphatesolubilizing bacteria and fungi in various cultivated and virgin alberta soils. Beyond the phosphate solubilization, many p solubilizing microorganisms increase the mycorrhizal root colonization by production of specific.
The use of coldtolerant phosphate solubilizing microorganisms to enhance crop productivity in mountainous regions is examined, as are the ecological diversity and biotechnological implications of phosphate solubilizing microorganisms. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphatesolubilizing bacteria, based upon visual observation. A wide range of soil fungi are reported to solubilize insoluble phosphorous such as aspergillus niger and. Phosphate is solubilized via organic acid synthesis and released by microorganisms puente et al. The present study focuses on the phosphatesolubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from banana plant, revealing the dominance of aspergillus species 234. The phosphate solubilization ability of the fungi was analyzed by determining the phosphate solubilization efficiency e of each isolate. Mineral phosphate solubilization several reports have examined the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate 38. Microbial phosphorus solubilization and its potential for. Some important cultural conditions were optimized, and optimal.
Isolation and characterization of two phosphatesolubilizing. Phosphate solubilizing microorganisms naturally occurring rhizospheric phosphorus solubilizing microorganism psm dates back to 1903 khan et al. Generally, the phosphatesolubilizing bacteria outnumber phosphate solubilizing fungi by 2 150 times kucey, 1983. Isolation and characterization of phosphate solubilizing. Introduction agriculture, occupies a unique position both conventional as. Phosphate solubilizing bacteria from sunflower rhizosphere 439 centrifuged at 10,000 rpm for 15 minutes. The present study focuses on the phosphatesolubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. Phosphate solubilizing microorganisms psm, phosphate solubilizing fungi psf, phosphates olubilizing bacteria psb, acid production, phosphorus p, p solubilization, hplc, p immobilization created date. Three levels of phosphate fertilizer 0, 20 and 40 mg kg1, four levels of inoculums phosphate solubilizing bacteria, arbuscular mycorrhizal fungi, combination of fungi and bacteria, and control and four harvest times 25, 50, 75 and 100 days after planting were used. Oct 26, 2015 a number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Phosphate solubilizing bacteria and fungi play an important role in p bioavailability and can be used to increase pue bolan et al.
Fig 1 growth parameter phosphate solubilizing fungi on some growth parameters,yield,p and n percentage in ground nut plants. Nov 09, 2015 in soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0. Novel phosphatesolubilizing bacteria enhance soil phosphorus. Pdf islam et al isolation of phosphate solubilizing fungi from subtropical soils9145applied ecology and environmental research 174. Collected rhizosphere soil samples were used for the isolation of phosphate solubilizing fungi on pikovskayas pkv agar medium, containing the following gl. The fact that all identified psolubilizing fungi belong to the aspergillus or pene. The analysis of phosphate solubilizing activity of the selected isolates were conducted qualitatively and quantitatively by plate screening method and broth culture method. Ability of fungi, isolated from nsukka peppers and garden. Inorganic phosphate solubilization by phosphate solubilizing fungi isolated from acidic soils. The phosphate solubilizing efficiency of isolated psb strain kupsb12 in pikovskayas broth indicated that the strain efficiently solubilized inorganic phosphate in the medium containing 0. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphate solubilizing bacteria, based upon visual observation. One fungal strain was found to be efficient phosphorus solubilizer in pikovskaya medium.
Isolation of phosphate solubilizing fungi from rhizosphere soil and its. Thus the application of p solubilizing fungi is recommended as a sustainable way for. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. Isolation and characterization of a phosphatesolubilizing. Phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. Two psf njdl03 and njdl12 were isolated and identified as penicillium oxalicum and. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Phosphate solubilizing activity is greater for fungi than bacteria.
Number of psb among total psm in north iranian soil was around 88 % fallah, 2006. This study aimed to isolate a highefficient level of phosphatesolubilizing fungus from rhizosphere soil samples phosphate mines liuyang county, hunan province, china and apply it in solubilization of rps. Evidence for naturally occurring phosphate solubilizing microorganism dates back to 1903 khan et al. Pdf plant growth promotion by phosphate solubilizing fungi. Fungi were isolated from the rhizosphere of pepper and gardenegg plants, with the aim of solubilizing the insoluble phosphate, identifying the most potent isolate from each rhizosphere and testing their tolerance to cadmium metal. In soil, phosphatesolubilizing bacteria constitute 1 50% and fungi 0. Apr 29, 2016 phosphate solubilizing fungi psf have huge potentials in enhancing release of phosphorus from fertilizer. In most bacteria, the production of organic acids is shown to be related to the dissolution of mineral glucose to gluconic acid ga as the foremost mechanism for mineral phosphate solubilization in gramnegative bacteria. Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates. Despite phosphorus being widely and abundantly distributed in the soil in both its inorganic and organic forms, many soils throughout. Oct 31, 20 in soil, psolubilizing fungi constitute about 0. Isolation, screening and characterization of phosphate.
A total of twenty two rhizospheric fungi were isolated from different healthy crop plants of hassan, karnataka, india and tested for its efficacy to solubilize. Frontiers identification and characterization of the. After confirming the phosphate solubilizing ability on solid medium, the phosphate. Plant growth promotion by phosphate solubilizing fungi current perspective. Isolation and characterization of two phosphatesolubilizing fungi. A number of phosphate solubilizing bacteria and fungi act as plant growth promoters because of their ability to release iaa souchie et al. Phosphate solubilizing microbes have been reported for plant growth promoting and enhancing yield kapoor, mishra, and kuhkreja 1989. Phosphorous and phosphate solubilising bacteria and their. Ability of fungi, isolated from nsukka peppers and gardenegg. Application of phosphate solubilizing fungi in the field has been reported to increase crop yield. Pdf phosphatesolubilizing fungi for efficient soil. Isolation and identification of potential phosphate.
The present study has been undertaken to isolate lignolytic and phosphate solubilizing fungi from arecanut husk waste. Effect of different biofertilizer inoculated with phosphate solubilizing fungi aspergillus niger on plant growth on free shipping on qualified orders. Phosphate solubilizing fungi isolated and characterized from teff. In contrast, 5 of the 33 genomes representing novel phosphatesolubilizing microbial taxa e. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %. This study aimed to isolate a highefficient level of phosphate solubilizing fungus from rhizosphere soil samples phosphate mines liuyang county, hunan province, china and apply it in solubilization of rps. Coinoculation of nitrogenfixing and phosphatesolubilizing. The bacterial cultures were positive for siderophore, hcn and ammonia. A total of 37 phosphate solubilizing microbial colonies were isolated on the pikovskayas agar medium, containing insoluble tri calcium phosphate tcp from agricultural soil.
To date, most representative strains of aspergillus spp. Moreover, psolubilizing fungi do not lose the p dissolving activity upon repeated sub culturing under laboratory conditions as occurs with the psolubilizing bacteria sperber 1958a, b. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. The type of crystals to neutral or calcareous soils whitelaw, 2000.
Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Microorganisms play an important role in the availability of soil phosphate to the root system and enhance the mobilization of phosphate in soil richardson, 2001. Role of phosphatesolubilizing microorganisms in sustainable. Optimization of phosphate solubilization by aspergillus. A number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Soil microorganisms play an important role in making the phosphorus available to plants by mineralizing the organic phosphorus in the soil.
Evaluation of phosphate solubilizing fungi from mangrove. Isolation of phosphate solubilizing bacteria and fungi from. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem. Some important cultural conditions were optimized, and optimal conditions for temperature, glucose. Based on morphological observations, 16s rrna sequencing, cellular fatty. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya pvk medium. A wide range of microorganisms are capable of degrading lignin present in the arecanut husk waste for their growth. Impact on growth and development of economically important plants. The selected strain was identified as aspergillus niger based on morphological and microscopic studies. Among the whole microbial population in soil, psb constitute 1 to 50 %, while phosphorus solubilizing fungi psf are only 0. Based on morphological observations, 16s rrna sequencing, cellular fatty acid composition analysis. The efficiencies of phosphate solubilization were investigated in two liquid media.
Pdf plant growth promotion by phosphate solubilizing. Pdf isolation of phosphate solubilizing fungi from rhizosphere soil. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Phosphatesolubilizing fungi psf generally enhance available phosphorus p released from soil, which contributes to plants p requirement.
A wide range of soil fungi are reported to solubilize insoluble phosphorous such as aspergillus niger and penicillium sp. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. On the other hand, with increases in nacl concentration the amount of p released among phosphate solubilizing fungi was found to decrease throughout the incubation periods srinivasan et al. It is important to explore a highefficient phosphate solubilizing approach with a kind of microorganisms. The study indicated that the bacteria have more solubilizing ability of. Phosphorus p is one of the major bioelements limiting agricultural production. The fungi isolates were obtained from the rhizosphere of pepper and gardenegg plants, using pvk agar and nbripbpb broth culture to. Inoculation with phosphatesolubilizing fungi diversifies. The high proportion of psm is concentrated in the rhizospheres and is known to be more metabolically active than those isolated from sources other than the rhizosphere vazquez et al. Pdf inorganic phosphate solubilization by phosphate. Phosphate solubilizing bacteria psb have the potential to enhance phosphate induced immobilization of metals to remediate contaminated soil. Phosphate solubilizing fungi, plackettburman, response surface methodology.
Fungi isolation phosphate solubilizing fungi psf were isolated from 25 subsamples of the soils using selective media, pikovskaya medium pvk. The isolated phosphate solubilizing fungi belonged to genera of aspergillus 55. Microorganisms play an important role in the propagation and growth of plants. Psb possess phosphate solubilizing ability and they can convert the insoluble phosphatic compounds. It is important to explore a highefficient phosphatesolubilizing approach with a kind of microorganisms. A total of 32 bacterial isolates including mesorhizobium n10, azotobacter n12 and phosphatesolubilizing bacteria n10 were isolated and tested for siderophore, hcn, ammonia, indole acetic acid production and phosphate solubilization in vitro. Jul 31, 2014 xiaoa cq, chib ra, huangb xh, zhangc wx, qiua gz, wanga dz 2008 optimization for rock phosphate solubilization by phosphatesolubilizing fungi isolated from phosphate mines. This reaction, appearing as a halo or clear zone on the plate, is used to assess the psolubilizing. Isolation of phosphate solubilizing bacteria and fungi. Isolation of phosphorussolubilizing fungus from soil to. In vitro solubilization of inorganic phosphate by phosphate.
Pdf phosphatesolubilizing fungi for efficient soil phosphorus. Plant growth promotion by phosphate solubilizing fungi taylor. Original article effects of phosphate solubilizing. Phosphate solubilizing bacteria an overview sciencedirect. Synergistic effect of a phosphate solubilizing fungus and an arbuscular mycorrhizal fungus on leucaena seedlings in an oxisol fertilized with rock phosphate. Propagation of plants through stem cuttings is a popular method widely practiced in hortic. Number of microbial species plays key role in p solubilization these includes bacteria, fungi, actinomycetes and even algae. Phosphorus p is an essential macronutrient for plant growth. Phosphatesolubilizing fungi for efficient soil phosphorus management article pdf available october 2017 with 418 reads how we measure reads.
In general, phosphate solubilizing fungi produce more acids and consequently exhibit greater phosphatesolubilizing activity than bacteria in both liquid and solid media venkateswarlu et al. The organic depends on the phosphate source and on the fungal strain. Phosphate solubilizing bacteria and their role in plant. Phosphate solubilizing fungi, mangrove soils, biofertilizer, aspergillus niger, penicillium sp. Organic acids production and phosphate solubilization by. Plant growth promotion by phosphate solubilizing fungi current perspective article pdf available in archives of agronomy and soil science 561 february 2010 with 2,282 reads. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. The acid and alkaline phosphatase activity were ranged from 8. A total of 359 fungal isolates were obtained from 150 rhizosphere soil samples of haricot bean, faba bean, cabbage, tomato, and sugarcane. A number of theories explain the mechanism of inorganic phosphate solubilization.
Effect of phosphate solubilizing fungi on growth and nutrient. In soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0. The colonies showing clear halo zones around the microbial growth were considered as phosphate solubilization. Phosphate solubilization potential of rhizosphere fungi. Five highly efficient psb strains, h22, y11, y14, y34, and s32, were screened and isolated from an alfalfa rhizosphere in heavy metalcontaminated reclamation area in shanxi province, china.
The application of isotopic 32p and 15n dilution technique to evaluate the interactive effect of phosphate solubilizing rhizobacteria, mycorrhizal fungi and rhizobium to improve the agronomic efficiency of rock phosphate form legume crops. Soil phosphate desorption induced by a phosphate solubilizing. The present study focuses on the phosphate solubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. Inoculation with phosphate solubilizing fungi diversifies the bacterial cornmunit. Accordingly, soil becomes phosphorus pdeficient, making p one of the most important nutrient elements limiting crop productivity.
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